What is the history of the thin prep method?
•Cytyc Corporation is a company engaged in the design, development, manufacture, and marketing of clinical products that focus on women’s health since 1987. In order to overcome limitations on conventional pap, the company introduced the ThinPrep Pap Test. In May 1996, the Food and Drug Administration (FDA) in the USA approved the ThinPrep 2000( T 2000 ) as a replacement for the conventional Pap smear method in USA for use in screening for the presence of atypical cells, cervical cancer or its precursor lesions (Low Grade Squamous Intraepithelial Lesions, High Grade Squamous Intraepithelial Lesions), as well as all other cytologic categories . dr.vikram saraswat |Saraswat Pathology in Agra
•The heart of the ThinPrep® System is the ThinPrep 2000 Processor, a semi automated slide preparation unit that produces remarkably uniform thin-layer slides, virtually free of obscuring artifacts such as blood, mucous and inflammation. Diagnosis, Diagnostics, and Efficient Treatment Plans,Pathologist.
Step 1
A gynecologic sample is collected using a broom-type or cytobrush/spatula cervical sampling device.
A gynecologic sample is collected using a broom-type or cytobrush/spatula cervical sampling device.
Step 2
Instead of smearing the cells on a slide, the sampling device is rinsed into a ThinPrep vial containing PreservCyt® transport medium(buffered methanol soln). The device is then discarded.
Instead of smearing the cells on a slide, the sampling device is rinsed into a ThinPrep vial containing PreservCyt® transport medium(buffered methanol soln). The device is then discarded.
Step 3
The sample vial is capped, labelled, and sent to the laboratory for slide preparation.
The sample vial is capped, labelled, and sent to the laboratory for slide preparation.
Step 4
At the laboratory, the vial is placed into the ThinPrep 2000 Processor. First, a gentle dispersion step breaks up blood, mucous, non-diagnostic debris, and then thoroughly mixes the sample. A negative pressure pulse is generated which draws fluid though a TransCyt® Filter( a micropore filter) that collects a thin, even layer of diagnostic cellular material. The ThinPrep 2000 Processor constantly monitors the rate of flow through the TransCyt Filter during the collection process to prevent the cellular presentation from being too scant or too dense. The filter is then removed and dabbed onto an electrically charged slide causing cells to transfer onto the glass slide. This is then stained in a separate process using same staining machine as is used for conventional samples. Pathology labs in agra - Dr.vikram saraswat
At the laboratory, the vial is placed into the ThinPrep 2000 Processor. First, a gentle dispersion step breaks up blood, mucous, non-diagnostic debris, and then thoroughly mixes the sample. A negative pressure pulse is generated which draws fluid though a TransCyt® Filter( a micropore filter) that collects a thin, even layer of diagnostic cellular material. The ThinPrep 2000 Processor constantly monitors the rate of flow through the TransCyt Filter during the collection process to prevent the cellular presentation from being too scant or too dense. The filter is then removed and dabbed onto an electrically charged slide causing cells to transfer onto the glass slide. This is then stained in a separate process using same staining machine as is used for conventional samples. Pathology labs in agra - Dr.vikram saraswat
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