dr.vikram saraswat


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Limitations of conventional pap smear

• Inadequate samples constitute about 8% of the specimens received. • False-negative results as high as 20-30% have been reported, which occurred due to clumping of cells when the cells are not uniformly spread on the glass slide. • Sometimes, other contents of the cervical specimen such as blood, mucus, bacteria and yeasts contaminate the sample and prevent the detection of abnormal cells. • If exposed to air for too long before being fixed on the slide, cervical cells can become distorted. • Human error is probably the primary threat to accurate interpretation. An average Pap smear slide contains 50,000-300,000 cells that must be examined and if the sample contains only a few abnormal cells within a crowded background of healthy cells, the abnormal cells may be missed. • Due to limited capacity, long back-logs for reporting cervical cytology were often seen. An automated system was needed to improve screening productivity and reduce manual workload.
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What is the history of the thin prep method?

•Cytyc Corporation is a company engaged in the design, development, manufacture, and marketing of clinical products that focus on women’s health since 1987. In order to overcome limitations on conventional pap, the company introduced the ThinPrep Pap Test. In May 1996, the Food and Drug Administration (FDA) in the USA approved the ThinPrep 2000( T 2000 ) as a replacement for the conventional Pap smear method in USA for use in screening for the presence of atypical cells, cervical cancer or its precursor lesions (Low Grade Squamous Intraepithelial Lesions, High Grade Squamous Intraepithelial Lesions), as well as all other cytologic categories .  dr.vikram saraswat |Saraswat Pathology in Agra •The heart of the ThinPrep® System is the ThinPrep 2000 Processor, a semi automated slide preparation unit that produces remarkably uniform thin-layer slides, virtually free of obscuring artifacts such as blood, mucous and inflammation.  Diagnosis, Diagnostics, and Efficient Treatment P...
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